V.V. Dokuchaev Soil Science Institute

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Estimation of dna quantity in different groups of microorganisms within genetic horizons of the dark-gray soil

A. D. Zhelezova, O. V. Kutovaya, V. N. Dmitrenko, A. K. Tkhakakhova, S. F. Khokhlov

V.V. Dokuchaev Soil Science Institute, 119117 Moscow, Pyzhevskii 7, bld. 2

Molecular-biological methods permitted to study the structure of the microbial community in the profile of dark-gray soil (Luvic Retic Greyzemic Phaeozem) in Kashira district of the Moscow region. Microorganisms playing an important role in transformation of the soil organic matter are mainly concentrated in the topsoil and the major microbiological studies are related to this part of the soil profile. However, the study of the microbial community in the lower soil horizons is not only of theoretical but also practical interest in view of increasing the intensity of erosion processes. The method of quantitative polymerase chain reaction was used to estimate the DNA quantity of bacteria, archaea and micromycetes in horizons of the above soil. The material of mole passage on a depth 80 cm was also studied. The highest DNA quantity of bacteria and archaea was found in the upper humus-accumulative horizon (9.6 × 108 and 9 × 107 copy/g of soil respectively). Its quantity was decreased downwards the profile, what is connected with changes in the physic-chemical conditions of soil. DNA of micromycetes was evenly distributed throughout the soil profile (5.4–9.4 ×
×107 copy/g). In the mole passage material the DNA content of different microorganism groups was close to that in lower mineral soil horizons. This may be explained by water infiltration through the mole passage material accompanying by eluviation of microorganisms in the period of soil wetting. The factors affecting the DNA amount of microorganisms are elementary soil processes including the biogenic-accumulative process in the upper soil horizons, clay-illuvial and humus-illuvial processes in the lower horizons of the dark-gray soil.

Keywords: Polymerase chain reaction, profile distribution of microorganisms, bacteria, micromycetes.

REFERENCES 

  1. Andronov E.E., Petrova S.N., Pinaev A.G., Pershina E.V., Rakhimgalieva S.Zh., Akhmedenov K.M., Gorobets A.V., Sergaliev N.Kh. Analysis of the Structure of Microbial Community in Soils with Different degrees of Salinization Using T-RFLP and Real-Time PCR Techniques, Eurasian Soil Science, 2012, Vol. 45(2), pp. 147-156. DOI: 10.1134/S1064229312020044.
  2. Andronov E.E., Pinaev A.G., Pershina E.V., Chizhevskaya E.P. Vydelenie DNK iz obraztsov pochvy (metodicheskie ukazaniya), (Solation of DNA from soil samples (guidelines)), St. Petersburg, 2011, 27 p.
  3. Bazykina G.S. Hydrological degradation of automorphic soils in agricultural landscapes, Byulleten Pochvennogo instituta im. V.V. Dokuchaeva, 2012, Vol. 70, pp. 43-55.
  4. Vasilenko Ye.S., Kutovaya O.V., Tkhakakhova A.K., Martynov A.S. Changes in the intensity of soil-biological processes caused by different-sized aggregates of migrationary-mycelial chernozems, Byulleten Pochvennogo instituta im. V.V. Dokuchaeva, 2014, Vol. 73, pp. е70-е81.
  5. Dobrovol'skii G.V. Raznoobrazie genezisa i funktsii lesnykh pochv, Pochvovedenie, 1993, No. 9, pp. 5–12.
  6. Edinyi gosudarstvennyi reestr pochvennykh resursov Rossii. Versiya 1.0. Kollektivnaya monografiya, (Unified State Register of soil resources of Russia. Version 1.0. collective monograph), Moscow, 2014. 768 p.
  7. Klassifikatsiya i diagnostika pochv Rossii (Classification and diagnosis of soil Russia). Smolensk, 2004, 342 p.
  8. Manucharova N.A. Molekulyarno-biologicheskie aspekty issledovanii v ekologii i mikrobiologii, (Molecular biological aspects of research in ecology and microbiology), Moscow, 2010. 47 p.
  9. Polyanskaya JI.M., Geidebrekht V.V., Stepanov A.JI., Zvyagintsev D.G. Ras-predelenie chislennosti i biomassy mikroorganizmov po profilyam zonal'nykh tipov pochv, Pochvovedenie, 1995, No. 3, pp. 322–328.
  10. Urusevskaya I.S., Sokolova T.A., Shoba S.A., Bagnavets O.S., Kuibyshe-va I.P. Morfologicheskie i geneticheskie osobennosti profilya svetlo-seroi lesnoi pochvy na pokrovnykh suglinkakh, Pochvovedenie, 1987, No. 4, pp. 5–16.
  11. Agnelli A., Ascher J., Corti G., Ceccherini M.T., Nannipieri P. Distribution of microbial communities in a forest soil profile investigated by microbial biomass, soil respiration and DGGE of total and extracellular DNA, Soil Biol. Biochem., 2004, pp. 859–868.
  12. Baldrian P., Vetrovsky T., Cajthaml T., Dobiasova P., Petrankova M., Snajdr J., Eichlerova I. Estimation of fungal biomass in forest litter and soil, Fungal ecology, 2013, pp. 1–11.
  13. Fierer N., Schimela J.P., Holden P.A. Variations in microbial community composition through two soil depth profiles, Soil Biol. Biochem., 2003, pp.167–176
  14. Gangneux C., Akpa-Vinceslas M., Sauvage H., Desaire S., Houot S., Laval K. Fungal. bacterial and plant dsDNA contributions to soil total DNA extracted from silty soils under different farming practices: Relationships with chloroform-labile carbon, Soil Biol. Biochem., 2011, pp. 431–437
  15. IUSS Working Group WRB. World Reference Base for Soil Resources. International soil classification system for naming soils and creating legends for soil maps. World Soil Resources Reports No. 106. FAO, Rome. 2014. 181 p.